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Dr. Simeonov, the author and inventor on over 15 peer-reviewed scientific publications and patents, comes to the center with a truly diverse background, ranging from bio-organic chemistry and molecular biology to microfluidic device research and development. Prior to joining NCGC in November 2004, Dr. Simeonov was a senior scientist at Caliper Life Sciences, a leading developer of microfluidic technologies, where he was responsible for both basic research on novel assay chemistries and development of microfluidic products.
Dr. Simeonov is a Literature Editor of Assay and Drug Development Technologies, a bi-monthly journal published by Mary Ann Liebert, Inc. His current research interests include novel detection chemistries and techniques, assay miniaturization, and novel approaches to screening.
Background:
- 1993-1998:Ph.D. in Bio-Organic Chemistry from the University of Southern California, Los Angeles, CA. Researched the mechanism of nitrogen fixation (Azotobacter vinelandii-based in vivo and in vitro systems). Laboratory of Prof. Charles E. McKenna.
Expertise: single-step synthesis and characterization of small explosive homologues of nitrogen reduction intermediates; enzymology; bacterial growth and protein purification; anaerobic techniques.
Selected publication:
- Simeonov, A. M. and McKenna, C. E. A Convenient Phase-Transfer Method for Preparation of Pure cis-Dimethyldiazene (cis-Azomethane) in Aqueous Solution. Proton and Carbon NMR Studies of trans- and cis-Dimethyldiazene. J. Org. Chem. 60, 1897-9 (1995).
- McKenna, C. E., Simeonov, A. M., Eran, H. and Bravo-Leerabhandh, M. Reduction of Cyclic and Acyclic Diazene Derivatives by A. vinelandii Nitrogenase: Diazirine and trans-Dimethyldiazene. Biochemistry 35, 4502-14 (1996).
- 1998-2000:: Postdoctoral fellow at the Scripps Research Institute, LA Jolla, CA. Discovered blue fluorescent antibodies which represent a new paradigm in small molecule-protein interactions. Performed mechanistic studies of catalytic antibody functions. Laboratory of Prof. Richard Lerner and Kim Janda.
Expertise: molecular biology; catalytic antibodies; protein purification; phage display; time-resolved and steady-state fluorescence techniques.
Selected publication:
- Datta, A., Wentworth Jr., P., Shaw, J. P., Simeonov, A., and Janda, K. D. Catalytically Distinct Antibodies Prepared by the Reactive Immunization Versus Transition State Analog Hapten Manifolds. J. Am. Chem. Soc. 121, 10461-7 (1999).
- Simeonov, A. M., et al. Blue-Fluorescent Antibodies. Science 290, 307-313 (2000).
- 2000-2004:: Caliper Life Sciences, Mountain View, CA. Developed novel fluorescence-based assays for high-throughput screening and clinical diagnostics.
Expertise: bio-conjugate chemistry; assay development; separation (chromatographic and electrophoretic) techniques; technology evaluation.
Selected publication:
- Simeonov, A. and Nikiforov, T., Single Nucleotide Polymorphism Genotyping Using Short, Fluorescently Labeled Locked Nucleic Acid (LNA) Probes and Fluorescence Polarization Detection. Nucleic Acids Res. 30, e91 (2002).
- Nikiforov, T. and Simeonov, A., Application of Fluorescence Polarization to Enzyme Assays and Single Nucleotide Polymorphism Genotyping: Some Recent Developments. Comb. Chem. High Throughput Screening 6, 201-12 (2003).
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